Coding

Part:BBa_K4219001

Designed by: Yoav Navon   Group: iGEM22_TAU_Israel   (2022-09-25)


Evolutionary-stabilized optimized eCFP coding device

This part is an optimized version of the BBa_J04421 construct, stored in the 15P position, plate 2 of the iGEM 2021 Kit Distribution - upon which an optimization process, taken out by the ESO software (developed by TAU representative group for the iGEM 2020 competition), was induced. The term "optimization" refers, in that context, to the evolutionary stability (and the expression levels) of the construct: improving the ability of it to preserve functionality over long periods of time by avoiding loss-of-function mutations. While designing this new part, the goal stood in front of our eyes was to induce minimal changes, so the functionality of the initial construct will be preserved, while still an improved stability of it might be formed. The users of that part should address it exactly the same as they were addressing the original one - use it as an eCFP coding device with an IPTG inducible promoter - while taking into account that it is evolutionary-stabilized, namely will preserve its functionality for longer periods.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1148
    Illegal EcoRI site found at 915
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 915
    Illegal SpeI site found at 1149
    Illegal PstI site found at 1163
    Illegal NotI site found at 7
    Illegal NotI site found at 1156
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 915
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1149
    Illegal EcoRI site found at 915
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal EcoRI site found at 915
    Illegal XbaI site found at 16
    Illegal SpeI site found at 1149
    Illegal PstI site found at 1163
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 264


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